Automated Sequence Generation and Data Prep Toolkit for LC-MS Intact Mass Analysis of Antibody Biologics
Published:
Context: Biopharmaceutical Industry
Date: May 2025
The Challenge High-throughput LC-MS intact mass analysis is essential for biologics screening, but its efficiency is hindered by tedious manual sequence setup and data prep steps, which slow workflows, increase errors, and limit throughput.
My Solution I developed a toolkit to significantly streamline this process, initially targeting our group’s specific LC-MS workflow. The toolkit is also designed to be adaptable for other similar assays with some modifications:
- Automated Sequence Generation:
Utilizes plate map information provided by users and automatically generates a CSV file for a generic LC-MS system to generate run sequence and another formatted for downstream deconvolution workflows. - User-Friendly Design:
GUI for non-programmers, auto-installs Python packages, customizable settings for naming and storage.
Impact
- Reduced a manual task from ~15 min to ~15 sec for a single sequence.
- Minimized human errors.
- Improved user experience.
Technologies Used
Python, Tkinter (GUI), CSV Processing